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. 2013 Jun 5;8(6):e65389. doi: 10.1371/journal.pone.0065389

Figure 3. Upregulation of oxidative stress-related proteins in DBA retinas with age and IOP.

Figure 3

A. Representative western blots and B. bar charts demonstrating oxidative stress-related protein expression in C57 and DBA retinas. GFAP did not change with age in C57 retinas (p>0.72), and did not increase significantly in DBA retinas until 10 months of age (211%; †p = 0.003), resulting in a 106% increase in GFAP in DBA retinas at this age (*p<0.001). With age Cp decreased in C57 retinas (36%; p>0.21) and increased in DBA retinas (147% to 203%; †p<0.007); as a result Cp levels in DBA retinas were increased 84% and 257% at 7 and 10 months of age, respectively, compared to C57 retinas (*p<0.001). HO-1 increased with age in both C57 (124% on average; p>0.13) and DBA retinas (316%; †p<0.001), resulting in levels 198% higher in 10 month DBA retinas compared to 10 month C57 retinas (*p<0.001). NOS-2 increased 94% in C57 (p>0.06) and 138% DBA retinas (†p = 0.03) with age. NOS-2 levels were increased in DBA retinas compared to C57 retinas at all ages examined (72% on average; p>0.07). RAGE did not change in C57 retinas (p>0.67) but did increase 44.5% in DBA retinas with age (p>0.214). As a result, RAGE increased 97% and 81% at 7 and 10 months compared to C57 retinas (p<0.006). (Inset) IOP of C57 (average over all ages) and DBA mice used for western blotting (n = 3). IOP was similar in C57 and DBA mice at 3 months of age, but due to a 48% increase in DBA IOP with age, was 42.5% higher on average in DBA mice compared to age-matched C57 mice (*p<0.001).