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. 2013 Jun 5;8(6):e63651. doi: 10.1371/journal.pone.0063651

Figure 2. Generation of 3D reconstructions.

Figure 2

A. Example of a 10 µm section from P. guttatus 10 days stained for myocardium with antibodies against rabbit cardiac troponin I. B. Same section as in A with annotations made in Amira®. C. 3D reconstruction based on 160 sections projected out of the section of A with mesenchyme and transparent lumen (myocardium not shown). D. Same 3D reconstruction as in C visualizing the annotated compartments. E. Five reconstructions exemplifying the transition from the youngest to the oldest stage.