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. 2013 Jun 5;8(6):e65538. doi: 10.1371/journal.pone.0065538

Figure 2. Expression of KLF5 in and its effect on sphere formation in RWPE-1 cells.

Figure 2

A. Detection of Ac-KLF5 and unAc-KLF5 in RWPE-1 spheres, along with marker CK5 for undifferentiated cells and AR for differentiated cells. B. Detection of total KLF5, Ac-KLF5 and unAc-KLF5 by IF staining in 3-D-cultured RWPE-1 cells (Day 1 in matrigel) with the knockdown of KLF5. C. Confirmation of KLF5 knockdown (upper panel) by Western blotting with antisera against KLF5 and the decrease in the number of spheres by the knockdown of KLF5. The same number of cells (6000) was seeded onto each chamber. After two-weeks of 3-D culture, the average number of spheres from 8 chambers per group was calculated.