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. 2013 Jun 5;8(6):e65538. doi: 10.1371/journal.pone.0065538

Figure 5. Confirmation of Klf5 deletion mediated by the expression of Cre in mouse prostates.

Figure 5

A. Breeding strategy for the production of mice with the three desired genotypes of Klf5. B. PCR-based genotyping of Klf5, floxed Klf5, and PB-Cre, with Il-2 as a control for the detection of Cre. C. Detection of Klf5 knockout in different tissues from 9-week-old heterozygous mice. Primers F and R1 amplify the wildtype allele while primers F and R2 amplify the knockout allele. Tissues in the lanes are: 1, anterior prostate; 2, dorsal prostate; 3, lateral prostate; 4, ventral prostate; 5, seminal vesicle; 6, urethra; 7, testis; 8, bladder; 9, heart; 10, brain; 11, lung; 12, liver; 13, stomach; 14, kidney; 15, spleen; 16, small intestine; 17, colon; 18, salivary gland; and 19, tail. D. Detection of Klf5 mRNA by real-time RT-PCR in whole prostates of 13-week-old mice with different deletion status of Klf5 (w/w, wildtype; f/w, heterozygous deletion; f/f, homozygous deletion). E. Reduced expression of Klf5 protein by the knockout of Klf5, as detected by IF staining in prostates from 16-week-old mice (Magnification: 200X).