. 2013 Jun 5;8(6):e65538. doi: 10.1371/journal.pone.0065538

Table 1. List of PCR primer sequences used in the generation of hybridization probes, genotyping and RT-PCR.

Description	Forward/reverse
Hybridization probes
5′	ACAAGAGCCATGAGTGCTTTTCCG/CTTTTAGAACATCAGGGATGCCACC
3′	TGTATAGGAAGGAGCAAGGGACCAC/TCGTTTTCATGCACAGGGTGATG
En	ATTTCTTGGGGTCTGCCAGTGTCG/CAGTCATCTTGCTATCTCCTGAGCG
Genotyping
*Klf5^wt*	ACAGATTTGAGGCAGTTTGGC/GGGCCAACTCCTAAGTGTTGC
*Klf5^mut*	ACAGATTTGAGGCAGTTTGGC/CAAATCCTGCCCTCACCCTG
*Cre*	CGGTCGATGCAACGAGTGAT/CCACCGTCAGTACGTGAGAT
*Il-2*	CTAGGCCACAGAATTGAAAGATCT/GTAGGTGGAAATTCTAGCATCATCC
Real-Time PCR
*Klf5*	ACTGCCCTCGGAGGAGCTGG/ATGCTCTGAAATTATCGGAACTG
*Gapdh*	CCAGCCTCGTCCCGTAGACA/GCCGTTGAATTTGCCGTGAG