Figure 1. Effect of malvidin on LPS induced activating phosphorylation of NFκB.
Total (phosphorylated and unphosphorylated) NFκB (t- NFκB) as well as the phoshorylated form of its p65 subunit (p-NFκB) was detected by immunoblotting of whole RAW 264.7 macrophage lysates after treating the cells for 1h as indicated. Actin was used as a loading control. Representative blots (A) and densitometric evaluations (B,C) of 3 independent experiments are shown. Pixel densities were normalized to that of the actin. Values are given as means ± SEM. *** p<0.001 compared to untreated control, ### p<0.001 compared to LPS alone.