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. 2013 Jun 5;8(6):e65355. doi: 10.1371/journal.pone.0065355

Figure 6. Effect of LPS and malvidin on MKP-1 expression in LPS treated RAW 264.7 macrophages.

Figure 6

Effect of LPS and malvidin on steady state MKP-1 protein level was assessed by immunoblotting from whole cell lysate after treating the cells as indicated for 1h. Actin was used as a loading control. Representative blots (A) and densitometric evaluations (B) of 3 independent experiments are shown. Pixel densities were normalized to that of the actin. MKP-1 mRNA expression (C) was determined in another aliquot of cells treated as above using Q-RT-PCR analysis. β-Actin was used as a housekeeping control gene. Specific primer sequences and PCR conditions are described in Materials and Methods. Values are given as means ± SEM. * p<0.05, *** p<0.001 compared to untreated control, ## p<0.01 ### p<0.001 compared to LPS alone.