Table 1. Effect of temperature on kinase activity of Pim-1 wild type and mutants.

	Wild type	Y53H	E124Q	E135K	E142D
E a ‡ (kcal/mol)	16.86±0.54	14.27±0.49	15.46±0.42	11.36±0.34	14.21±0.39
Tmax (°C)	37	35	35	30	37
Kinase activity at Tmax (s−1)	0.098	0.018	0.040	0.015	0.056

E _a ^‡ was determined by the Arrhenius equation (Eqn. 1) in the temperature range between 10°C and the optimal temperature of each protein.