Table 1. Classification of pathogen-specific sequence reads from symptomatic and non-symptomatic samples.
Category of sequence reads | Reads from ribo-depletedcDNA library | Reads from dual-depletedcDNA library | ||
Symptoms | No Symptoms | Symptoms | No Symptoms | |
Reads mapped to specific virus | ||||
GRLaV | 15,036 | 0 | 406 | 0 |
GFLV | 14,383 | 0 | 978 | 0 |
GRSPaV | 662 | 1,353 | 42 | 68 |
Reads mapped to specific viroid | ||||
GYSVd-1 | 13,914 | 9,004 | 987 | 490 |
CEYVd | 1,605 | 152 | 5 | 10 |
CEVd | 856 | 86 | 6 | 2 |
HpSVd | 1,964 | 2,980 | 164 | 185 |
Other* | 5,568 | 2,693 | 644 | 1,604 |
Total | 53,988 | 16,268 | 3,232 | 2,539 |
GRLaV = Grapevine redleaf-associated virus, GRSPaV = Grapevine rupestris stem pitting-associated virus, GFLV = Grapevine fanleaf virus, HpSVd = Hop stunt viroid, GYSVd-1 = Grapevine yellow speckle viroid 1, CEVd = Citrus exocortis viroid, CEYVd = Citrus exocortis Yucatan viroid.
Reads mismapped to virus- or viroid-like sequences.
Classification and abundance of high-throughput sequence reads obtained from ribo-depleted and dual-depleted cDNA libraries were determined by mapping the reads onto the virus/viroid database using mapping software BWA 0.6.