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. 2001 Jun 1;21(11):3839–3848. doi: 10.1523/JNEUROSCI.21-11-03839.2001

Fig. 1.

Fig. 1.

The Exocyst complex exhibits distinct subcellular localization on neuronal differentiation. Monoclonal antibody against the Exocyst complex subunit Exo70 was produced as explained in Materials and Methods. A, Western blot analysis using the anti-Exo70 monoclonal antibody 70X13F3 showed that it recognized a single band in brain lysate (lane 1). Recombinant Exo70 was used as a molecular weight control (lane 2). Thearrow indicates a degradation product of the recombinant Exo70. B, C, The subcellular localization of the Exocyst complex in both undifferentiated (B) and differentiated (C; NGF; 50 ng/ml) PC12 cells was determined by immunofluorescence microscopy. PC12 cells were fixed with 100% methanol, and the anti-Exo70 antibody was visualized with anti-mouse antibodies conjugated to FITC.B, The Exocyst complex exhibited a perinuclear localization in undifferentiated PC12 cells (arrowheads). C, In differentiated PC12 cells, the Exocyst complex is found in the cell body (arrowheads), along the neurite, and at the growth cone (arrows). Scale bars, 50 μm.