Figure 2.DACT2 expression is associated with promoter hypermethylation in primary HCCs. (A) Representative MSP results of DACT2 in primary HCCs. (B) DACT2 expression in a representative HCC case. Left image: adjacent non-tumor tissue (100×) and the indicated fields enlarged (400×). Right image: tumor tissue (100×) and the indicated fields enlarged (400×). (C) DACT2 expression scores are shown as box plots, the horizontal lines represent the median score; the bottom and top of the boxes represent the 25th and 75th percentiles, respectively; the vertical bars represent the range of data. Expression of DACT2 was different between non-tumor tissues and tumor tissues in 62 matched primary HCCs. **p < 0.01. (D) The correlation of DACT2 hypermethylation and expression level were analyzed in 62 matched primary HCCs. *p < 0.05. (E) β-catenin expression in the representative methylated case (400×). β-catenin expression is positive mostly in the membranes of hepatic cells (left image) and in the cytoplasm and nucleus in tumor cells (right image).