Table 1. Association of clinical factors with DACT2 methylation and expression in HCC patients.
| Clinical factors | n | DACT2 Methylation (%) | p (χ2) | Loss of expression (%) | p (χ2) |
|---|---|---|---|---|---|
| Age (years) |
|
|
1 |
|
0.01 |
| < 50 |
21 |
12 (57.14) |
|
17 (80.95) |
|
| ≥ 50 |
41 |
22 (53.65) |
|
18 (43.9) |
|
| Gender |
|
|
1 |
|
0.27 |
| Male |
54 |
30 (55.55) |
|
32 (59.26) |
|
| Female |
8 |
4 (50.00) |
|
3 (75.00) |
|
| Virus infection |
|
|
0.6 |
|
0.27 |
| HBV |
49 |
28 (57.14) |
|
28 (57.14) |
|
| HCV |
2 |
1 (50.00) |
|
2 (100.00) |
|
| No |
11 |
5 (45.45) |
|
5 (45.45) |
|
| AFP (ng/ml) |
|
|
1 |
|
0.04 |
| < 500 |
44 |
24 (54.54) |
|
21 (47.73) |
|
| ≥ 500 |
18 |
10 (55.55) |
|
14 (77.78) |
|
| Tumor size (cm) a |
|
|
0.79 |
|
0.7 |
| < 8.07 |
36 |
19 (52.77) |
|
21 (58.33) |
|
| ≥ 8.07 |
26 |
15 (57.69) |
|
14 (53.84) |
|
| ALTSG stage |
|
|
1 |
|
1 |
| T1,T2 |
15 |
8 (53.33) |
|
8 (53.33) |
|
| T3,T4 |
47 |
26 (55.32) |
|
27 (57.44) |
|
| AJCC stage |
|
|
0.17 |
|
0.78 |
| T1,T2 |
36 |
24 (66.67) |
|
24 (66.67) |
|
| T3,T4 |
21 |
10 (47.62) |
|
11 (52.38) |
|
| Differentiation |
|
|
0.96 |
|
0.67 |
| Poor |
10 |
6 (60.00) |
|
6 (60.00) |
|
| Poor to moderate |
8 |
4 (50.00) |
|
3 (37.50) |
|
| Moderate |
36 |
20 (55.56) |
|
20 (55.56) |
|
| Moderate to well |
3 |
2 (66.67) |
|
2 (66.67) |
|
| Well |
5 |
2 (40.00) |
|
4 (80.00) |
|
| Cirrhosis |
|
|
0.77 |
|
0.57 |
| No |
16 |
8 (50.00) |
|
8 (50.00) |
|
| Yes |
46 |
26 (56.52) |
|
27 (58.70) |
|
| Capsule invasion b |
|
0.315 |
|
|
0.1 |
| No |
39 |
20 (51.28) |
|
25 (64.10) |
|
| Yes |
20 |
13 (65.00) |
|
8 (40.00) |
|
| Encapsulated b |
|
|
1 |
|
0.30 |
| No |
12 |
6 (50.00) |
|
5 (41.67) |
|
| Yes |
31 |
16 (51.61) |
|
20 (64.52) |
|
| Vascular invasion |
|
|
0.54 |
|
0.53 |
| No |
49 |
28 (57.14) |
|
29 (59.18) |
|
| Yes | 13 | 6 (46.15) | 6 (46.15) |
a The average of tumor size of all 62 samples is 8.07 cm. bInformation on capsule invasion and encapsulation is only available for 59 and 43 samples, respectively.