Figure 4. Analysis of methylation status of RASSF1 promoter in cervical SCC samples and cell lines. Schematic representation of results of sodium bisulfite sequencing: numbers under the horizontal line indicate CpG dinucleotides within RASSF1 promoter, the broken arrow – transcription initiation site; black circle,methylated CpG; white circle, unmethylated CpG; gray circle, partially methylated CpG; each row of circles represents a cloned DNA molecule for all samples excepting SiHa and number 16T, where the total PCR products are presented; T, tumor; Nc, normal cervix. Numbers of tumors correspond to Table 4.