Figure 3. PTEN suppresses PLK-1 and γ-tubulin by inhibiting the activation of Akt. (A) PTEN was silenced in NHEKs followed by immunoblot analysis for the indicated proteins. Quantitation of the change in proteins levels is graphed in the right panel. Error bars represent s.d. from three separate experiments. (B) NHEKs were transfected with non-silencing control (NSC) or PTEN specific siRNA followed by treatment with 10 μM MK2206 or DMSO control for 16 h. Immunoblot analysis for the indicated proteins was performed, and the change in total protein levels is plotted in the right panel. Error bars represent s.d. from three separate experiments.