Figure 4. Treatment-selective transcriptional cooperation between p53-inducing stimuli and estradiol. qPCR reactions for the 16 chosen genes were performed using 384-well plates in a final volume of 10 µl using TaqMan^® Gene Expression Assays with 3 biological and 2 technical replicates for each condition. GAPDH, B2M and β-actin housekeeping genes served as internal controls. Asterisks indicate statistically significant, more than additive effects in the combined treatment as described in the “Materials and Methods.” The same RNAs used in the microarray experiments were tested in (A and B), where the experiment served also as a validation of the array results, while all results in (C) were obtained from independent treatment and RNA extractions.