Figure 2. Effect of LOX-PP on MK number and MK maturation marker gene expression level. (A) Bone marrow cells were isolated and cultured as described in Figure 1A. Percentage of MKs was based on CD41-FITC staining with FACS analysis. (B) Total MK number was calculated based on cultured BM cell number and % MK from (A). Data are averages of ± SD of five experiments. Statistical analysis was applied using the Student’s t-test. No statistical significance was found, p > 0.05. C. qRT-PCR of isolated MKs 9, see “Materials and Methods”), pre-treated with either control or 10 µg/ml LOX-PP to evaluate CD41 and PF4 mRNA levels. Data are averages of ± SD of three experiments. Statistical analysis was applied using the Student’s t-test. No statistical significance was found, p > 0.05.