A, Cells were seeded at equal density in 6-well plates, grown in the absence of EGF for 10 days, and then stained with crystal violet to visualize cell viability and proliferation.
B, Cells were seeded at equal density in 6-well plates, grown in the absence of EGF for 16 days, and counted on days 1, 6, 11, and 16. Error bars represent SEM of triplicate samples. Results are representative of 5 independent experiments. Please see Supplemental Table S1 for cell line nomenclature.