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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Cancer Res. 2013 Apr 11;73(11):3248–3261. doi: 10.1158/0008-5472.CAN-12-1578

Figure 1. Double mutant KRAS and PIK3CA cells show synergistic growth effects in EGF-independent condition.

Figure 1

A, Cells were seeded at equal density in 6-well plates, grown in the absence of EGF for 10 days, and then stained with crystal violet to visualize cell viability and proliferation.

B, Cells were seeded at equal density in 6-well plates, grown in the absence of EGF for 16 days, and counted on days 1, 6, 11, and 16. Error bars represent SEM of triplicate samples. Results are representative of 5 independent experiments. Please see Supplemental Table S1 for cell line nomenclature.