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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Cancer Res. 2013 Apr 10;73(11):3381–3392. doi: 10.1158/0008-5472.CAN-12-4047

Figure 3. Inhibition of the hedgehog pathway may augment EGFR driven EMT-like state in EGFR dependent cell lines.

Figure 3

A. EGFR signaling activation by the EGF ligand (100ng/ml) generates an EMT-like phenotype in HN11 and Tu-167 cells that is completely blocked by erlotinib (1μM) but not IPI-926 (1μM). B and C. EGF increases cellular motility in EGFR dependent cells which was blocked by erlotinib but augmented by IPI-926. D. EGF increased expression of pro-EMT genes ZEB1 and VIM as well as suppressed E-cad generating an EMT-like gene expression profile in HN11 and Tu-167 cells but not 584 cells. However, E-CAD expression was suppressed by erlotinib treatment in 584 cells. *P<0.05, **P<0.01.