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Determination of gene silencing efficiency using siRNA gene expressing cassettes. A) Determination of siRNA expression from the U6-siRNA DNA cassettes by co-transfection. Upper two panels: H1299 and MCF-7 cancer cells were transfected with p-EGFP-c3, without or with co-transfection with U6-eGFP siRNA or U6-control siRNA expressing nanocassettes for 48 h. B) MCF-7 GFP gene stable cells were transfected with U6-eGFP siRNA or U6-Control siRNA nanocassettes for 48 h.
