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. Author manuscript; available in PMC: 2014 Jun 10.
Published in final edited form as: Small. 2013 Jan 6;9(11):1964–1973. doi: 10.1002/smll.201201973

Figure 4.

Figure 4

uPAR-targeted delivery and gene silencing efficiency of luciferase siRNA-expressing DNA nanocassettes in human tumor cell lines. A) Targeted delivery of Luc siRNA expressing DNA cassettes into human cancer cells. B) Efficiency of gene silencing in cancer cells. Luciferase activity in firefly luciferase gene stably transfected human breast cancer MCF-10DCIS cell lysates was measured at 24 h following nanoparticle incubation. Luciferase units from the no treatment cell lysate serves as 100%. C) Comparison of gene silencing efficiency of delivery of unconjugated RNA-based siRNAs with targeted nanoparticles carrying siRNA expressing DNA nanocassettes. Cells were cultured in 96-well plates and then incubated with 20 pmol of luciferase siRNA (Invitrogen) or an equal molar DNA concentration of ATF-IONP-Luc siRNA expressing cassettes. Luciferase activity in the wells was measured 48 h following the incubation using the Xenogen IVIS system. Luciferase activity of untreated cells serves as 100%.