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. Author manuscript; available in PMC: 2014 Jun 10.
Published in final edited form as: Small. 2013 Jan 6;9(11):1964–1973. doi: 10.1002/smll.201201973

Figure 6.

Figure 6

Targeted silencing of survivin gene expression in human tumor cells A) Internalization of hATF-QD-Survivin siRNA expressing DNA cassettes by MDA-MB-231 breast cancer cells. B) Western blot analysis. Following treatment, cell lysates were then collected for Western blot analysis using antibodies against survivin or caspase 3. Activation of caspase 3 is shown as the detection of the cleaved caspase 3 fragments (low molecular weight bands at 17 kDa). C) Effect of nanoparticle delivery of Survivin-siRNA nanocassettes. Left panel: Fluorescent images show internalization of hATF-QD-Survivin siRNA nanocassettes in MIA PaCa-2 pancreatic cancer cells. Red: QD signal. Right panel: Crystal violet cell proliferation assay 2 days following treatment. O.D. value of no treatment group was used as 100%.