Fig. 1.

Clotting times for pooled plasma mixed with phosphate buffered saline (PBS). Citrate anticoagulated pooled human plasma was mixed with PBS and incubated with thromboplastin-coated microspheres at a count of 10³, 10⁴, or 10⁵ mL⁻¹ for 3 minutes at 37°C prior to recalcification (8.3 mM, final Ca²⁺ concentration). The time for plasma to clot was recorded on a KC4 coagulation analyzer (A). Clotting time elongation was calculated for diluted plasmas by normalizing their clotting times to clotting times obtained with non-diluted plasma (B). Experiments were performed in duplicate, with the mean clotting time reported. The bar graphs represent the mean clotting time and clotting time elongation (n=3) and the error bars represent standard error of the mean.