Fig. 2.

Clotting times for pooled plasma mixed with plasma immunodepleted of specific coagulation factors. Citrate anticoagulated pooled human plasma was mixed with plasma immunodepleted of either FVII (A), FX (B), FIX (C), FVIII (D), FV (E), or FII (F) and incubated with thromboplastin-coated microspheres at a count of 10³, 10⁴, or 10⁵ mL⁻¹ for 3 minutes at 37°C prior to recalcification (8.3 mM, final Ca²⁺ concentration). The time for plasma to clot was recorded on a KC4 coagulation analyzer. Clotting times elongation was calculated for mixed plasmas by normalizing their clotting times to 100% pooled plasma clotting times. Experiments were performed in duplicate, with the mean clotting time reported. The bar graphs represent the mean clotting time (n=3) and the error bars represent standard error.