Effects of specific blockers of TRPC channels on calcium influx and PRL release. A, Inhibition of spontaneous calcium influx by SKF96365 in lactotrophs. B, Dose-dependent effect of SKF96365 on basal PRL release. From top to bottom: 10, 25, 50 μM SKF96365. C, Dose-dependent effects of 2-APB on basal PRL release. Left, representative records in response to 0, 5, 10, 25, 50, 75 and 100 μM 2-APB (from top to bottom). Right, concentration-dependence study revealed the IC50 value of 38 μM. Mean ± SEM values of four experiments are shown.