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(A) Schematic of the in vitro flow chamber. A flow-focusing microfluidic device produced microbubbles in situ. Calcein was administered alongside the microbubbles, and a single element ultrasound transducer insonated the microbubbles as they flowed by confluent rat aortic smooth muscle cells. Microbubble (B) production rate and (C) diameter as a function of the FFMD liquid flow rate (pressure was held constant at 55.2 kPa).
