Skip to main content
. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Neurobiol Dis. 2013 Apr 29;0:95–103. doi: 10.1016/j.nbd.2013.04.015

Figure 3. Effect of recombinant Ngb (rNgb) on OGD-induced mPTP opening in isolated mitochondria.

Figure 3

Figure 3

(A) Characterization of rNgb with SDS-PAGE and Western blot. (B) Isolated mitochondria was incubated with rNgb (90 ug/ml), CsA (10μM) or rNgb+CsA for 30 min followed by 30 min OGD plus 10 min reoxygenation. Relative NAD+ content in mitochondria was measured in each group (n=4, * P<0.05). (C) Cyt c contents in isolated mitochondria and releases into media after OGD were measured by Western blot (n=3, * P<0.05).