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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2012 Sep 28;136:264–270. doi: 10.1016/j.jsbmb.2012.09.022

Figure 2. CYP24 is differentially expressed in primary human lung adenocarcinomas that harbor EGFR or K-ras mutations.

Figure 2

Gene expression values were abstracted for the 20 K-ras mutation-positive lung adenocarcinomas and 127 EGFR mutation-positive lung adenocarcinomas included in the whole genome, gene expression profiling study of Okayama et al., (GSE31210) [21]. Each point represents an individual case. Data were analyzed using the Bioconductor packages in the R statistical computing environment microarray data processing. Specifically, the RMA function was used to generate expression summary values through convolution background correction, quantile normalization, and a summarization based on a multi-array model fit using the medial polish algorithm. The Limma program was used to calculate the level of differential expression based on the log2 transformed gene expression value. Multiple testing was corrected using Benjamin and Hochberg’s algorithm. Box edges indicate the 25%-ile and 75%-ile of CYP24 expression for each population. Horizontal bars indicate the median CYP24 expression value.