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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Clin Cancer Res. 2013 Apr 2;19(11):2984–2994. doi: 10.1158/1078-0432.CCR-12-2008

Figure 1.

Figure 1

A. MEDI-573 inhibits bot IGF-1 and IGF-2 stimulation of Akt phosphorylation. Sarcoma cells were grown for 24 hr under serum-free conditions, then incubated for 5 - 60 min with MEDI-573 (30μg/ml), or 5 min without antibody, and stimulated for 5 min with IGF-1 (10 ng/ml) or IGF-2 (50 ng/ml). B. MAB391 inhibits only IGF-1 not IGF-2 stimulation of Akt phosphorylation. Sarcoma cells were grown for 24 hr under serum-free conditions, then incubated for 5 - 60 min with MAB391 (15μg/ml), or 5 min without antibody, and stimulated for 5 min with IGF-1 (10 ng/ml) or IGF-2 (50 ng/ml). Cells were harvested 5 min after IGF-stimulation at the times shown and phosphorylation of Akt(Ser473) and total Akt determined by immunoblotting.