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Binding of LD22-4 to U87MG cells. Competition binding studies were performed with 2.5 μM biotinylated-LD22-4 in the absence (A) or presence of 20-fold (B) or 60-fold (C) unlabeled LD22-4. Fluorescence images are shown in the upper panels and transmission images are shown in the bottom panels. In separate experiments, binding studies were performed with 2.5 μM biotinylated-LD22-4 (D) or biotinylated-LD63-6 (E). Magnification: 100X.
