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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Cancer Res. 2013 May 10;73(11):3316–3325. doi: 10.1158/0008-5472.CAN-12-3015

Figure 4.

Figure 4

Binding of LD22-4 to NRP1 expressing HEK293 cells. A. The expression of NRP1 by HEK293-NRP1 cells was confirmed by FACS analysis (α-NRP1). Biotinylated-LD22-4 at 0, 100, 1000 nM was incubated with the cells and the presence of biotinylated-LD22-4 was assessed by incubating cells with fluorescein-conjugated streptavidin. The cells were analyzed by FACS analysis for specific LD22-4 binding which fell within the gated region (M1). A. native HEK293 cells; B. HEK293-NRP1 cells. B. Biotinylated-LD22-4 was incubated with HEK293-NRP1 and native HEK293 cells and bound LD22-4 visualized by fluorescence microscopy.