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. 2013 May 7;4:1814. doi: 10.1038/ncomms2811

Figure 4. dmd-1 is required for maintenance of presumptive male germ cells in asexuals.

Figure 4

(a) qPCR showing decreased nanos transcript levels in dmd-1(RNAi) versus control asexuals. Error bars indicate 95% confidence intervals calculated based on s.e.m., n=8 individual animals. (b) Loss of gH4+/nanos+ germ cell clusters (asterisks) in dmd-1(RNAi) asexuals. gH4+/nanos− cells in dmd-1(RNAi) animals are somatic neoblasts. Images shown are single confocal optical sections; gH4 and nanos mRNAs are detected by two-colour FISH. (c) qPCR showing that dmd-1 transcript levels in nanos(RNAi) versus control asexuals remain unchanged. Error bars indicate 95% confidence intervals calculated based on s.e.m., results are the mean from three independent experiments using pools of nine planarians. Scale bars: b, 50 μm.