Figure 5. dmd-1 is required for male germ cell specification.

(a) Experimental scheme for generating animals devoid of germ cells and reproductive structures. Only animals that had successfully regenerated (indicated by presence of pharynx) were scored. (b) By 14 days post amputation, planarians devoid of germ cells regenerate dorsolateral nanos-positive cells; dmd-1(RNAi) animals fail to regenerate dorsolateral nanos-positive cells. Images shown are single confocal sections; nanos transcripts are detected by FISH. Scale bars: b, 50 μm.