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. 2013 May 7;4:1807. doi: 10.1038/ncomms2795

Figure 1. Effects of GFP-tagging of Cdc42 on cell growth and polarization.

Figure 1

(a) Growth of the standard lab strains S288C and W303 and the arrest-strain RWS116 at different temperatures. Shown are the respective control strains (no tag) and strains where Cdc42 has been tagged at its endogenous locus (end) or as additional copy integrated in the ectopic LEU2 locus (ect). (b) Western blot of different Cdc42 fusion proteins in the strains used in (a). Cdc42 was detected with a polyclonal serum against Cdc42. A monoclonal antibody against the constitutively expressed glyceraldehyde-2-phosphate dehydrogenase (GAPDH) was used as loading control. Identity of visualized bands is indicated on the right. (c) Quantification of Cdc42 expression levels showing fold change compared with expression of the endogenous copy in the respective strain background. Bars correspond to mean±s.d. from three independent experiments. Labels correspond to untagged Cdc42 in strains expressing GFP-Cdc42 at the endogenous locus (end Cdc42) or the LEU2 locus (ect Cdc42), to GFP tagged Cdc42 at the endogenous locus (GFP-Cdc42) and GFP-myc tagged Cdc42 at the LEU2 locus (GM-Cdc42). Strain backgrounds are indicated on x axis.