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. 2013 Jun;68(6):825–833. doi: 10.6061/clinics/2013(06)17

Figure 1.

Figure 1

MiR-424 and miR-381 are poorly expressed in 786-O cells, and the combination of miR-424 and miR-381 significantly inhibited the proliferation of 786-O cells. (A) Cell survival curves of 786-O cells treated with a combination of miR-424 and miR-381 and with either miR alone. The lines represent three independent experiments, which were performed in triplicate. Synergistic inhibition was significant in 786-O cells, and cell survival was decreased by approximately 4-fold compared with the control (p<0.01). (B) No similar effects were observed in the normal cell line HK-2. (C) Both miRs (miR-424 and miR-381) were analyzed with real-time PCR using total RNA from 786-O and HK-2 cells. We calculated ΔCt (miR Ct-U6 Ct) from the Ct values of each gene for normalization. The ΔCt values were then converted into relative gene expression levels using the 2−ΔΔCt method. The graph shows the relative fold difference in gene expression between 786-O and HK-2 cells. **p<0.01.