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. 2013 Jun;68(6):825–833. doi: 10.6061/clinics/2013(06)17

Figure 2.

Figure 2

MiR-381 and miR-424 synergistically promoted mitotic entry and induced apoptosis. (A) The mitotic index (%) was determined in both 786-O cells and HK-2 cells. The combination of miRs significantly increased the mitotic index. (B) Cell death was analyzed through caspase-3/7 activation in cells treated with miR-424, miR-381, the combination, or DMSO for 48 h. Caspase 3/7 activity increased only in the presence of both miRs compared with the control. (C) Cell lysates collected from control cells and cells treated with miR-424, miR-381, and the combination of miR-381 and miR-424 were assessed with SDS-PAGE. The combined treatment strongly reduced the level of p-Cdc2, increased the level of pHH3, and increased the percentage of mitotic cells without DNA damage repair, which caused mitotic catastrophe and apoptosis. The combination of miRs also down-regulated Bcl-2 expression compared with the control cells. β-Actin was used as a loading control. (D) and (E) The fraction of apoptotic 786-O cells was investigated after the cells were stained with both Annexin V-FITC and PI. (D) The means ± SDs of three independent experiments. (E) Representative results. *p<0.05, **p<0.01.