FIGURE 7.

cAMP uptake via Cx26 hemichannels. (A) Schematic of the experimental environment for HeLaCx26/SpIH cells. (B) Current recorded from a HeLa Cx26/SpIH-expressing cell. Perfusion with the Ca²⁺-free KAsp external solution induced a significant SpIH current increase over time. (C) Average of current densities measured from 5 cells: 6.4 ± 2.2 versus 32.3 ± 3.3 pA/pF, before and after perfusion, respectively; P = 0.008. 500 μM of cAMP was present in the external solution throughout the entirety of the experiment.