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. 2013 Jun 6;4:75. doi: 10.3389/fphar.2013.00075

FIGURE 9.

FIGURE 9

Local hemichannel activation. (A) Schematic illustrating an experiment with local application of cAMP. The perfusion pipette containing NaCl (*) or KAsp (**) solutions was brought close to the cell and a positive pressure was applied to allow the contents of the pipette to reach the cell membrane. Local application of cAMP via the external pipette did not affect the SpIH current in HeLaCx43/SpIH cells when the perfusion pipette contained the NaCl solution (*):6.6 ± 1.5 and 6.9 ± 1.3 pA/pF, before and during local cAMP application, respectively; n = 6, P = 0.456 (B). However, the SpIH currents did increase when the pipette contained the KAsp solution (**) (C). (D) Summary of current densities: 7.0 ± 1.6 and 23.9 ± 6.2 pA/pF, before and during local cAMP application, respectively; n = 6, P = 0.041. Currents marked in red in (B) and (C) correspond to recordings during the local application of cAMP; asterisks (*) and (**) indicate moments when a positive pressure was applied to the perfusion pipette.