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G6PD knockdown downregulates G6PD status. (a) g6pd mRNA level (relative to ef-1α:elongation factor 1α) was determined by real-time PCR. (N=4, P<0.005) (b) G6PD activity was measured by enzymatic assay and protein expression was determined by western blot (inlet). (N=8, P<0.005) **P<0.005 indicates significant difference between mock and G6PD-RNAi knockdown (Gi) C. elegans
