Figure 3.
G6PD knockdown enhances oxidative stress and DNA oxidative damage in C. elegans. (a) The ROS level in C. elegans was determined by incubating staged adults with DCFDA and the fluorescence was detected by fluorescent microplate reader. The fluorescence of G6PD-RNAi knockdown (Gi) C. elegans was subtracted by background and normalized with mock (N=6, P=0.038). (b) The DNA oxidative damage (8-OHdG) in C. elegans was determined by isolating DNA from staged adults followed by separation by HPLC and detection by electrochemical detector. The 8-OHdG ratio was displayed as the number of 8-OHdG molecules per 106 dG. (N=6, P=0.005) *P<0.05 indicates significant difference between mock and G6PD-RNAi knockdown (Gi) C. elegans