Figure 5.

Short-term H₂O₂ treatment affects germ cell apoptosis and egg production in C. elegans. (a) The apoptotic germ cells in the gonad of adult hermaphrodite with or without H₂O₂ treatment were determined. (b) Fluorescent microscopy images of germ cell apoptosis between mock and G6PD-RNAi knockdown (Gi) C. elegans with or without short-term 5 mM H₂O₂ treatment. White arrows indicate apoptotic germ cells in the gonad, scale bar=50 μm. (c) The egg production of young adult hermaphrodite with or without H₂O₂ treatment was determined. The black bar represents egg production from day 1 to day 2 post-treatment, and the white bar represents egg production from day 3 to day 4 post-treatment. The stack bar represents total egg production post-treatment. *P<0.05, **P<0.005 indicate significant difference compared with untreated mock. ^#P<0.05, ^##P<0.005 indicate significant difference compared with untreated G6PD-RNAi knockdown (Gi) C. elegans. These results were representative of three separate experiments (N=3)