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. 2013 May 16;4(5):e632. doi: 10.1038/cddis.2013.140

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Copyright © 2013 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/

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Manipulation of IP₃R2-expression levels in OCI-LY-1 and SU-DHL-4. (a) Western-blot analysis of IP₃R2 and IP₃R1 proteins in siCtrl- and siIP₃R2-transfected SU-DHL-4 is shown in the upper panel. Representative dot plots from flow cytometry analysis of apoptosis induced by 2 h treatment without or with 10 μM TAT-IDP^S in mock-, siCtrl-, and siIP₃R2-transfected SU-DHL-4 are shown in the middle panel. Quantitative analysis of three independent experiments of the TAT-IDP^S-induced apoptosis is shown in the bottom panel. (b) Western-blot analysis of IP₃R2 and IP₃R1 proteins in empty vector- and IP₃R2 expressing plasmid-transfected OCI-LY-1 is shown in the upper panel. Representative dot plots from flow cytometry analysis of apoptosis induced by 2 h treatment without or with 10 μM TAT-IDP^S in mock-, empty vector-, and IP₃R2-expressing plasmid-transfected OCI-LY-1 cells are shown in the middle panel. Quantitative analysis of three independent experiments of the TAT-IDP^S-induced apoptosis is shown in the bottom panel. Results are shown as average±S.D.