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Cytometric analyses for CD133 expression and side population profile. (a) Expression levels of CD133 were ∼3.9%. The purity of sorted populations for CD133 was routinely 90%. (b) SP cells were detected in A549 cells, with a percentage of about 12.3% of the total cell population, and the SP cell fraction was abolished in the presence of verapamil. The purity of sorted populations for side population was routinely 90%
