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RT-PCR, densitometry and western blot analyses of OCT4. (a) RT-PCR and densitometry evaluation showed an increase of OCT4 in all sorted and unsorted A549 cells except in CD133− A549 cells, after TGF-β1 treatment. (b) Western blot analyses showed an increase of OCT4 in all sorted and unsorted A549 cells except in CD133− A549 cells, after TGF-β1 treatment. β-Actin is used as loading control. *P<0.001, **P<0.0005, compared to the parental cell line (day 0 of treatment)
