Figure 4.

Effect of Aβ treatment on metabotropic purinergic Ca²⁺ signalling in EC and hippocampal astrocytes from non-Tg and 3xTg-AD mice. (a and b) Representative traces of ATP-induced Ca²⁺ responses in hippocampus and EC in control cells and in Aβ-treated cells derived from non-Tg mice, and control and Aβ-treated cells derived from 3xTg-AD mice (n=30 cells for each experimental protocol). The cells were exposed to 100 nM of Aβ for 72 h and then loaded with Fluo4-AM, and stimulated with 100 μM ATP for 15 s. (c) Mean values for integrals of [Ca²⁺]_i transients induced by ATP stimulation (n=150 cells from five different cultures for each experimental protocol)