Figure 6.

Induced adipogenic differentiation of NCI-H446 cells. The Oil Red O staining showed that when cultured in adipogenic induction/maintenance medium for two cycles, the cancer cells could produce a lot of lipid droplets: (a) the control group and (b) showing lipid droplets in induced cancer cells. (c) The magnified image of the cells in b, showing these lipid droplets more obviously. (d) Inducing for three cycles, these cancer cells collapsed due to accumulation of too much lipid droplets in the cytoplasm. (e) The phase contrast images showed bright lipid droplets accumulated in the mature adipocyte-like cancer cells, inducing for two cycles. Western blotting showed that after inducing differentiation, the cells overexpressed C/EBPβ, PPARγ, FAS, and adiponectin (Figure 5e). Scale bar, 20 μm (a and b); 5 μm (c–e)