Figure 5.
TLR4 in vitro agonist LPS shows a strong radio-protective role in vivo. (a) C57BL/10 mice (20 g in weight, N=20 per group) were injected with LPS at 2.5 mg/kg body weight and then 24 h later subjected to 7, 9 or 13 Gy TBI as indicated. Control mice received PBS. Survival was recorded. (b) Linear regression analysis of the survival rate for mice treated with LPS or PBS after IR. The equation and the correlation coefficient for the line are given. The calculated DRF using these equations was 1.29 (LPS treated mice versus PBS treated mice). (c) LPS protects both C57BL/10 mice and BALB/c mice from radiation injury. (d) WT C57BL/10 mice were exposed to 9 Gy of γ-irradiation at 1 Gy/min and then reconstituted with 2 × 105 of BMC from C57BL/10 mice treated with or without LPS (2.5 mg/kg) 24 h previously. *P<0.05