Figure 4.

Effect of IL-32β on CD8⁺ cytotoxic T cell and CD57⁺ NK cell number in tumor and spleen tissues, and blood of athymic nude mice inoculated with IL-32β-trasfected colon and prostate cancer cells. (a) Effect of IL-32β on the number of CD8⁺ cytotoxic T cells and CD57⁺ NK cells in tumor and spleen tissue section of IL-32β-transgenic mice, as determined in immunohistochemistry. (b) Effect of IL-32β on the number of CD8⁺ cytotoxic T cells and CD57⁺ NK cells in tumor and spleen tissue sections of athymic nude mice inoculated with IL-32β-trasfected colon and prostate cancer cells, as determined in immunohistochemistry. The images shown in (a) and (b) are representative of three sections from each mouse (n=3). (c and d) Subpopulation of immune cells determined after FACS analysis, as described in Materials and Methods section. The values in each area are the average subpopulation of immune cells (NK and CD8⁺ cells) *Significant difference from nontransgenic mice (*P < 0.05).