Figure 5.

Stimulation with PRE084 counteracts the downregulation of NF-κB pathway induced by mutant huntingtin proteins. (a and b) Immunoblot and quantification. The levels of NF-κB-p65 were downregulated by expression of 120Q-huntingtin and restored by 0.3 μM PRE084. β-actin was used as control. Values represent mean±S.D., n=3. **P<0.01 for 120Q versus C and *P<0.0 for PRE084+120Q versus 120Q. (c) NF-κB reporter assay using the luciferase reporter plasmid was performed as described in the Materials and methods. NF-κB activity was decreased in cells expressing FL mutant huntingtin protein (75QFL) and this was restored by addition of 0.3 μM PRE084. Progesterone stimulating also Sigma-2 receptors did not have the same effect. Values represent mean±S.D., n=3. *P<0.05 for 75QL versus C and for PRE084+75QFL versus 75QFL as well as for Progesteron+75QFL versus C