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. 2013 May 30;4(5):e648. doi: 10.1038/cddis.2013.173

Figure 5.

Figure 5

hCNT1 overexpression activates Akt and Erk. NP-9 and NP-29 cells were infected with AdhCNT1 or Adctrol at an MOI of 10. (a) The time-course of Akt and Erk phosphorylation was analysed in total cell extracts by western blotting using the indicated antibodies. (b) Infected NP-9 and NP-29 cells were treated with 50 μℳ PD98059 (MEK inhibitor) or 20 nℳ (+) or 100 nℳ (#) wortmannin (PI3K inhibitor) for 48 h. Total-cell lysates were analysed by immunoblotting with the indicated antibodies. (c) After infection with Adctrol (black bars) and AdhCNT1 (gray bars), NP-9 and NP-29 cells were treated with different concentrations of PD98059 and cell viability was assessed by MTT assay 72 h later. Data are expressed as percentage of viable cells relative to Adctrol- or AdhCNT1-infected cells. Results are means±S.E.M. (n=3). Statistical significance was determined with Student's t-test; ***P<0.005