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. 2013 Mar 19;23(6):803–819. doi: 10.1038/cr.2013.42

Figure 6.

Figure 6

FBW7-mediated KLF2 degradation was required for teratoma growth and vascular development. (A) Procedure for the teratoma analysis. (B) FBW7-mediated KLF2 degradation is essential for the teratoma growth in nude mice. As indicated, the ES cells as indicated were injected subcutaneously into nude mice. Mice were fed with doxycycline for 5 weeks. The teratomas were harvested. (B and C) Teratomas were collected and the expression of FBW7 and KLF2 was analyzed using western blotting (B) and qRT-PCR (C). Tubulin was used as loading control. (D) The size of the tumors was measured every 7 days. Five mice were inoculated in each group, and data in the graph represent the mean ± SEM (n = 5, *P < 0.05). (E) Teratoma sections were stained with HE or CD31 for blood vessel. (F) Quantification of blood vessels in teratoma. IOD, Integrated Optical Density. *P < 0.05; **P < 0.01; NS, not significant.